MACSfectin™ Reagent enables optimized transfection of plasmid DNA for a wide range of adherent and suspension cells in immunology, cancer, stem cell, and neuroscience research. Additionally, MACSfectin Reagent supports tranfection with RNA such as mRNA or siRNA. This unique transfection reagent is based on a novel class of cationic lipopolyamines designed to optimize nucleic acid condensation and augment the cytoplasmic release of the nucleic acid cargo. Additionally, good transgene expression with high cell viability is achieved due to MACSfectin Reagent’s excellent biodegradation characteristics.
Applications
MACSfectin Reagent can be applied for mammalian cell transfection using plasmid DNA, mRNA, and siRNA. Possible downstream applications after transfection with MACSfectin Reagent are protein expression and purification, protein interaction studies, reporter gene assays, fluorescent imaging, gene knock-down, and generation of stable cell lines.
10 adherent cell lines (A) and 6 suspension cell lines (B) were transfected with a plasmid vector expressing cell surface human CD4 in 6-well plates with MACSfectin Reagent and a competitor product using standard transfection conditions (4 µg DNA and 8–16 µL MACSfectin Reagent).
The number of transfected cells and mean fluorescence intensity (MFI) were assessed 48 hours post-transfection by staining the cells with CD4-APC and flow cytometric analysis using a MACSQuant
®
Analyzer. The MFI value of cells transfected with MACSfectin Reagent were set to 100% for comparison with the competitor product.
Efficient transfection using MACSfectin™ Reagent.
10 adherent cell lines (A) and 6 suspension cell lines (B) were transfected with a plasmid vector expressing cell surface human CD4 in 6-well plates with MACSfectin Reagent and a competitor product using standard transfection conditions (4 µg DNA and 8–16 µL MACSfectin Reagent).
The number of transfected cells and mean fluorescence intensity (MFI) were assessed 48 hours post-transfection by staining the cells with CD4-APC and flow cytometric analysis using a MACSQuant
®
Analyzer. The MFI value of cells transfected with MACSfectin Reagent were set to 100% for comparison with the competitor product.
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