YILIMART - Mouse IL-4 (25 μg)

IL-4 stands for interleukin 4. Mouse IL-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-4 can be used for a variety of applications, including:

In vitro differentiation of naive CD4⁺ T cells towards T_H 2 cells.
In vitro studies of bone marrow precursor development to DCs, together with GM-CSF.
In vitro investigation of B cell-T cell interaction, together with CD40-Ligand.

Background information

IL-4 is produced mainly by activated T

_H

2 cells and, to a lesser extent, by T

_H

1 cells. It binds to the IL-4 receptor α (CD124), followed by the dimerization with other receptor chains to generate type 1 and type 2 receptors. IL-4 promotes the proliferation and differentiation of activated B cells and the expression of MHC class II antigens. Mice over expressing IL-4 have elevated levels of IgE and IgG1 and show a deficiency in T cell maturation. CD4

⁺

T cells from knockout mice lacking IL-4 are not able to produce T

_H

2 cytokines after

in vitro

stimulation. The biological activity of IL-4 is species-specific, i.e. murine IL-4 is inactive on human cells and

vice versa.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Biological activity

Proliferation of HT-2 cells (NIBSC 91/656)
premium grade: ≥ 8×

10
⁶
U/mg
research grade: ≥ 5×

10
⁶
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Mouse IL-4, premium grade. The peak corresponds to the calculated molecular mass of 13683 Da.

Mass spectrometry analysis (ESI-MS) of Mouse IL-4, premium grade. The peak corresponds to the calculated molecular mass of 13683 Da.
Figure 2
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Figure 2

Mouse IL-4 biological activity.
Activity of Mouse IL-4, premium grade, (red bar) was compared to another commercially available product (black bar).

Mouse IL-4 biological activity.
Activity of Mouse IL-4, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 3
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Figure 3

SDS-PAGE of Mouse IL-4, premium grade under reducing (R) and non-reducing (NR) conditions.

SDS-PAGE of Mouse IL-4, premium grade under reducing (R) and non-reducing (NR) conditions.
Figure 4
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Figure 4

Mouse IL-4 activity assay:
The specific activity is determined by proliferation assay using mouse HT-2 cells. Activity of Mouse IL-4, premium grade (red line) was compared to another commercially available product (black line) with equivalent outcome.

Mouse IL-4 activity assay:
The specific activity is determined by proliferation assay using mouse HT-2 cells. Activity of Mouse IL-4, premium grade (red line) was compared to another commercially available product (black line) with equivalent outcome.