The Mitochondria Extraction Kit - Tissue is designed to accelerate and simplify the isolation of mitochondria from human and mouse tissue. The kit has been developed for use with the gentleMACS Dissociators. The resulting tissue homogenates provide the ideal basis for subsequent magnetic labeling and isolation of intact, functional mitochondria at high yield.
Background information
The Mitochondria Extraction Kit - Tissue allows the preparation of homogenates from the most common tissue types, e.g., muscle, heart, liver, brain, or kidney. The kit works in combination with gentleMACS Dissociators and integrates tissue homogenization and cell lysis in a single step. The tissue homogenates can be further processed with the Mitochondria Isolation Kit, human, or Mitochondria Isolation Kit, mouse tissue.
Downstream applications
Isolated mitochondria can be further analyzed by Western blots
1,6
or functional assays, including measurement of O
2
consumption, membrane potential, ATP
4,5,7
, or respiratory control
4
. Isolated mitochondria are well suited for mitochondrial RNA expression profiling
Isolation of mitochondria from human and mouse tissue.
Isolation of mitochondria from human and mouse tissue.
Selected references
Sacchi, S. et al. (2011) Evidence for the interaction of d-amino acid oxidase with pLG72 in a glial cell line. Mol. Cell. Neurosci. 48(1): 20-28
Bandiera, S. et al. (2011) Nuclear outsourcing of RNA interference components to human mitochondria. PLoS One 6(6): e20746
Barrey, E. et al. (2011) Pre-microRNA and mature microRNA in human mitochondria. PLoS One 6(5): e20220
Minet, A.D. and Gaster, M. (2011) The dynamic equilibrium between ATP synthesis and ATP consumption is lower in isolated mitochondria from myotubes established from type 2 diabetic subjects compared to lean control. Biochem. Biophys. Res. Commun. 409(4): 591-595
Eriksen, M. B. et al. (2011) Intact primary mitochondrial function in myotubes established from women with PCOS. J. Clin. Endocrinol. Metab. 96(8): E1298-1302
Guo, T. et al. (2010) Quantitative proteomics discloses MET expression in mitochondria as a direct target of MET kinase inhibitor in cancer cells. Mol. Cell. Proteomics 9(12): 2629-2641
Minet, A.D. and Gaster, M. (2010) ATP synthesis is impaired in isolated mitochondria from myotubes established from type 2 diabetic subjects. Biochem. Biophys. Res. Commun. 402(1): 70-74
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