Recombinant human IL-1β (interleukin 1 beta) can be used for maturation of Mo-DCs and for differentiation within the lymphoid lineage, such as Th17 development and B cell proliferation. The proinflammatory cytokine is produced upon infection or injury and is involved in diverse cellular processes. Developed for cell culture, functional assays, and differentiation studies, the recombinant human IL-1β is ideal for use.
Applications
Human IL-1β can be used for a variety of applications including:
Induction of Mo-DC maturation.
Chemotaxis assays.
Investigation of IL-1 receptor signaling.
Alternative names
IL1F2
Background information
Interleukin 1β (IL-1β) is a proinflammatory cytokine that is secreted mainly by monocytes and macrophages. IL-1β secretion has also been reported for a variety of other cells, including B cells, NK cells, dendritic cells, astrocytes, and microglial cells. It mediates inflammatory responses in B cells, T cells, and NK cells by inducing the production of cytokines, such as IL-2, IL-3, IL-6, as well as interferons. Upon exposure to IL-1β, endothelial cells and smooth muscle cells synthesize prostaglandins and other derivatives of arachidonic acid. In addition, IL-1β is found in synovial fluid of arthritis patients, causing degranulation of basophils and eosinophils as well as activation of osteoclasts. IL-1β is mitogenic for mesangial cells, glial cells, and keratinocytes. It has been shown that IL-1β is a potent modulator of CD40L-induced cytokine secretion by human dendritic cell (DC) subsets, such as monocyte-derived dendritic cells (MoDCs), CD34
+
-derived DCs, and peripheral blood DCs.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
The biological activity of Human IL-1β, premium grade is determined by proliferation assay using D10.G4.1 cells.
Human IL-1β activity assay.
The biological activity of Human IL-1β, premium grade is determined by proliferation assay using D10.G4.1 cells.
Selected references
Cunin P. et al. (2011) The tachykinins substance P and hemokinin-1 favor the generation of human memory Tʜ17 cells by inducing IL-1β, IL-23, and TNF-like 1A expression by monocytes. J. Immunol. 186(7): 4175-4182
Poole, S. and Gaines-Das, R. E. (1991) The international standards for interleukin-1 alpha and interleukin-1 beta. Evaluation in an international collaborative study. J. Immunol. Methods 142: 1-13
Dussiau, C. et al. (2015) Targeting IRAK1 in T-cell acute lymphoblastic leukemia. Oncotarget 6: 18956-18965
Bacher, P. et al. (2014) Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi. Mucosal Immunol 7(4): 916-928
Schipper H. S. et al. (2010) A multiplex immunoassay for human adipokine profiling. Clin. Chem. 56(8): 1320-1328
Kitamura, T. et al. (1991) IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoetic cell line, TF-1. Int. Immunol. 3: 571-577
Luft, T. et al. (2002) IL-1β enhances CD40 ligand-mediated cytokine secretion by human dendritic cells (DC): a mechanism for T cell-independent DC activation. J. Immunol. 168: 713-722
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