Recombinant human IL-6 (interleukin 6) can promote differentiation of B and T cells (Th17) and can stimulate proliferation of hematopoietic progenitor cells or hybridoma cells. The pleiotropic cytokine has crucial function, such as in the regulation of immune and inflammatory responses or hematopoiesis. Recombinant human IL-6 is specifically developed for use in cell culture, differentiation studies, and functional assays.
Applications
Human IL-6 can be used for a variety of applications, including:
Induction of colony formation from hematopoietic progenitor cells in semi-solid medium.
Replacement of feeder cells in the preparation of murine and human hybridomas.
In vitro differentiation of TH 17 cells.
Background information
IL-6, originally identified as a B cell differentiation factor, is a multifunctional cytokine, which regulates immune responses, hematopoiesis, acute phase responses, and inflammatory reactions. It induces, for instance, the terminal maturation of activated B cells into antibody-secreting plasma cells and acts in synergy with IL-3 to support the proliferation of hematopoietic stem cells. IL-6 is produced by many cell types, such as monocytes, fibroblasts, endothelial cells, T cells, etc. Disturbed IL-6 production has been associated with pathological processes including inflammatory autoimmune diseases, such as rheumatoid arthritis and cancer.
Quality description
Research-grade
cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade
cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).
Biological activity
Proliferation of B9 hybridoma cells (NIBSC 89/548)
Mass spectrometry analysis (ESI-MS) of Human IL-6, premium grade. The peak corresponds to the calculated molecular mass of 20812 Da.
Mass spectrometry analysis (ESI-MS) of Human IL-6, premium grade. The peak corresponds to the calculated molecular mass of 20812 Da.
Selected references
Kaebisch R. et al. (2014) Helicobacter pylori cytotoxin-associated gene A impairs human dendritic cell maturation and function through IL-10-mediated activation of STAT3. J. Immunol. 192: 316-323
Laurenti, E. et al. (2015) CDK6 levels regulate quiescence exit in human hematopoietic stem cells. Cell Stem Cell 16(3): 302-313
Ghirelli, C. et al. (2010) Systematic cytokine receptor profiling reveals GM-CSF as a novel TLR-independent activator of human plasmacytoid predendritic cells. Blood 115(24): 5037-5040
Gaines-Das, R.E. and Poole, S. (1993) The international standard for interleukin-6. Evaluation in an international collaborative study. J. Immunol. Methods 160: 147-153
Dighe N. et al. (2014) Long-term reproducible expression in human fetal liver hematopoietic stem cells with a UCOE-based lentiviral vector. PLoS One 9(8): e104805
Wright, C. et al. (2012) Detection of multiple autoantibodies in patients with ankylosing spondylitis using nucleic acid programmable protein arrays. Mol. Cell. Proteomics 11(2): M9.00384
Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
ex vivo
models of X
0
-linked, AR22
0
- and AR47
0
- chronic granulomatous diseases.
Biores Open Access 3(6): 311-326
Steinleitner, K. et al. (2012) EVI1 and MDS1/EVI1 expression during primary human hematopoietic progenitor cell differentiation into various myeloid lineages. Anticancer Res. 32(11): 4883-4889
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