YILIMART - Neural Tissue Dissociation Kit

The Neural Tissue Dissociation Kits have been developed for the gentle and efficient generation of single-cell suspensions from neural tissues.

The Neural Tissue Dissociation Kit - Postnatal Neurons has been optimized for the special needs of sensitive postnatal neurons during dissociation.

Background information

In less than an hour, the gentle two-step enzymatic dissociation procedure efficiently yields high numbers of viable cells. The Neural Tissue Dissociation Kit (P) contains papain, and the Neural Tissue Dissociation Kit (T) contains trypsin.

Some antigen epitopes are damaged by papain, others by trypsin. The kit based on papain is best suited for use with Anti-Prominin-1 MicroBeads, CD11b (Microglia) MicroBeads and Anti-A2B5 MicroBeads, whereas, for example, Anti-GLAST (ACSA-1) MicroBead Kit and Anti-PSA-NCAM MicroBeads require the use of the trypsin-based kit.

Please see figure 1 for further information with regard to epitope sensitivity and kit selection.

Figure 1
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Figure 1

Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.

Antigen compatibility of Dissociation Kits. For many applications, including MACS Cell Separation, it is crucial to conserve the integrity of particular cell surface epitopes during tissue dissociation. The table shows which kits conserve which specific epitopes. Epitope sensitivities have been tested using Miltenyi Biotec's antibodies if available.

Selected references
1. Környei et al. (2007) Astroglia-derived retinoic acid is a key factor in glia-induced neurogenesis. FASEB J. 21: 2496-2509
2. Lee, J. K. et al. (2008) Regulator of G-protein signaling 10 promotes dopaminergic neuron survival via regulation of the microglial inflammatory response. J. Neurosci. 28: 8517-8528
3. Skog, J. et al. (2008) Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat. Cell Biol. 10(12): 1470-1476
4. Szulwach, K. E. et al. (2010) Cross talk between microRNA and epigenetic regulation in adult neurogenesis. J. Cell Biol. 189: 127-141
Brochures and posters

Neuroscience research
Scientific posters

A novel approach for efficient dissociation of distinct adult mouse brain regions and subsequent sorting of neurons and neural stem cells using MACSQuant
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Tyto
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Semi-automated tissue dissociation and preserved epitope integrity optimize immunomagnetic sorting of neural cells
Customer reports and application notes

Dissociation of mouse brain to isolate microglia
Special protocols

Isolation of endothelial cells from mouse neonatal brain

Dissection of retinas

Schwann cell isolation from rat peripheral nerve tissue biopsies

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Neural Tissue Dissociation Kit - Trypsin

For research use only

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Background information

Figure 1

Figure 1

Selected references

Brochures and posters

Scientific posters

Customer reports and application notes

Special protocols

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