YILIMART - 7-AAD Staining Solution

7-AAD Staining Solution is a ready-to-use reagent suitable for the evaluation of cell viability in mono- or multiparametric analyses of human peripheral blood using flow cytometry.

7-AAD (7-amino-actinomycin D) is a fluorescent dye that intercalates into double-stranded DNA (GC rich regions). It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells. Therefore, it can be used instead of propidium iodide (PI) for the evaluation of cell death and apoptosis.

The fluorescence emission maximum for 7-AAD is at 647 nm. When excited at 488 nm, 7-AAD is detected in the red fluorescence channel commonly used for R-phycoerythrin (PE)-Cy

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5 tandem dye detection, with minimal spectral overlap into the yellow fluorescence channel commonly used for PE detection.

Background information

7-AAD (7-amino-actinomycin D) is a fluorescent dye that intercalates into double-stranded DNA (GC rich regions). It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells. Therefore, it can be used instead of propidium iodide (PI) for the evaluation of cell death and apoptosis.

The fluorescence emission maximum for 7-AAD is at 647 nm. When excited at 488 nm, 7-AAD is detected in the red fluorescence channel commonly used for R-phycoerythrin (PE)-Cy

^®

5 tandem dye detection, with minimal spectral overlap into the yellow fluorescence channel commonly used for PE detection.

Technical specifications

Product format: Reagents are supplied in phosphate-buffered saline, pH 7.2, at a concentration of 52.5 μg/mL.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates:

Figure 1

Aged human EDTA-treated whole blood samples (6 days old) were stained with 7-AAD Staining Solution using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant

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Analyzer. For analysis, white blood cells were gated based on forward and side scatter properties and after exclusion of cell debris and red blood cells based on scatter signals. Dead and apoptotic cells were identified by 7-AAD fluorescence.

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7-AAD Staining Solution

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Background information

Technical specifications

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