Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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A leukapheresis sample was stimulated with staphylococcal enterotoxin B for 16–20 hours. The sample was labeled with MACS ® GMP CD137-PE and MACS GMP CD8-APC and CD45-FITC for gating purposes. Cells were analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |