YILIMART - MACS GMP CD62L-PE

MACS

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GMP CD62L Fluorescent Antibodies consist of a mouse monoclonal antibody against the human CD62L antigen conjugated to a fluorochrome. They are developed for flow cytometric identification and flow cytometric sorting of CD62L-positive cells.

Alternative names

L-selectin, LAM-1, Leu-8, LECAM1, MEL-14, LEU8, TQ1

Disclaimer

MACS GMP Products are for research use and

ex vivo

cell culture processing only, and are not intended for human

in vivo

applications. For regulatory status in the USA, please contact your local representative.

Background information

Clone 145/15 recognizes the human CD62L antigen. Most hematopoietic cells express CD62L, including many peripheral blood B cells, T cells, monocytes, granulocytes, and some myeloid cells from bone marrow, and thymocytes. CD62L is continuously cleaved from the cell surface of neutrophils and lymphocytes by endoproteases. CD62L is important for homing of naive lymphocytes via endothelial venules to peripheral lymph nodes and Peyer’s patches.

Applications

MACS

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GMP CD62L Fluorescent Antibodies are for identification of CD62L-positive cells by flow cytometry as well as cell enrichment by flow cytometric sorting.

Quality statement

MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.

Figure 1

A leukapheresis sample was labeled with MACS

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GMP CD62L-PE, CD3-VioGreen™ and MACS

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GMP CD8-APC for gating purposes. Cells were analyzed by flow cytometry using the MACSQuant

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Analyzer 10. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Viable lymphocytes were further gated on CD3

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cells.

Brochures and posters

MACS GMP Tyto Consumables flyer
Scientific posters

GMP-compliant flow cytometric cell sorting of antigen-specificT cells using MACS
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GMP CD8-APC and MACS GMP CD137-PEFluorescent Antibodies on the MACSQuant
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Tyto
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Cell Sorter. Christiane Siewewrt
et. al. CIMT (2019)
Special protocols

MACS GMP Protocol cell sort on MACSQuant Tyto
Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

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MACS GMP CD62L-PE

For research use and ex vivo cell processing only

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Alternative names

Disclaimer

Background information

Applications

Quality statement

Figure 1

Brochures and posters

Scientific posters

Special protocols

Certificates

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