NK MACS GMP Medium (Phenol Red) has been optimized for the cultivation, activation, and expansion of isolated human NK cells or NK cells from peripheral blood mononuclear cells (PBMCs). It is manufactured without animal-derived components. NK MACS GMP Medium (Phenol Red) is filled in flexible bags, making handling in GMP confirming processes easy.
Disclaimer
MACS GMP Products are for research use and
ex vivo
cell culture processing only, and are not intended for human
in vivo
applications. For regulatory status in the USA, please contact your local representative.
Background information
NK MACS GMP Medium is serum- and xeno-component free.
Flexible bags (2000 mL)
Manufactured under strictly controlled conditions
Consistent lot-to-lot performance
Quality control: functionality test on every batch
Applications
NK MACS GMP Medium has beed developed for the cultivation and expansion of human NK cells from PBMCs or isolated human NK cells.
Quality statement
MACS GMP Products are manufactured and tested under a quality management system (ISO 13485) and are in compliance with relevant GMP guidelines. They are designed following the recommendations of USP <1043> on ancillary materials.
NK cells in NK MACS GMP Medium (Phenol Red). NK cell fold expansion from PBMCs (n=3) using 5% AB serum and 500 IU/mL of IL-2 and 140 U/mL IL-15 during 14 days showed high fold expansion rates for both, NK MACS Medium (light purple circle) and NK MACS GMP Medium (Phenol Red) (dark purple square).
NK cells in NK MACS GMP Medium (Phenol Red). NK cell fold expansion from PBMCs (n=3) using 5% AB serum and 500 IU/mL of IL-2 and 140 U/mL IL-15 during 14 days showed high fold expansion rates for both, NK MACS Medium (light purple circle) and NK MACS GMP Medium (Phenol Red) (dark purple square).
NK cell cytotoxicity assay. NK cells from PBMCs were expanded for 14 days in NK MACS Medium (light purple circle) and NK MACS GMP Medium (Phenol Red) (dark purple square) and were analyzed via flow cytometry for cytotoxicity against cell line K-562 at different effector-to-target (E:T) ratios.
NK cell cytotoxicity assay. NK cells from PBMCs were expanded for 14 days in NK MACS Medium (light purple circle) and NK MACS GMP Medium (Phenol Red) (dark purple square) and were analyzed via flow cytometry for cytotoxicity against cell line K-562 at different effector-to-target (E:T) ratios.
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