Figure 1
The differentiation potential of MSCs was assessed for adipocytes (A), chondrocytes (B), and osteoblasts (C). MSCs were cultivated in StemMACS AdipoDiff Medium for18 days and adipocyte differentiation was analyzed using a lipid-staining dye (red) to demonstrate lipid accumulation. Nuclei were detected with Dapi (blue).
After 26 days of culture in StemMACS ChondroDiff Medium, chondrocyte differentiation was analyzed using an anti-aggrecan antibody (red) for detection of proteoglycan. Nuclei were detected with Dapi (blue). After 10 days of culture in StemMACS OsteoDiff Medium, osteoblast differentiation was analyzed by detection of alkaline phosphatase activity.
The differentiation potential of MSCs was assessed for adipocytes (A), chondrocytes (B), and osteoblasts (C). MSCs were cultivated in StemMACS AdipoDiff Medium for18 days and adipocyte differentiation was analyzed using a lipid-staining dye (red) to demonstrate lipid accumulation. Nuclei were detected with Dapi (blue).
After 26 days of culture in StemMACS ChondroDiff Medium, chondrocyte differentiation was analyzed using an anti-aggrecan antibody (red) for detection of proteoglycan. Nuclei were detected with Dapi (blue). After 10 days of culture in StemMACS OsteoDiff Medium, osteoblast differentiation was analyzed by detection of alkaline phosphatase activity.