Cells sorted on the MACSQuant
® Tyto
® Cell Sorter remain inside the closed MACS
® GMP Tyto Cartridge at any time during the sort process. The cartridge consists of three compartments that can each be accessed through individual, aseptic Luer-Locks from above: the input chamber, the positive collection chamber and the negative collection chamber. Cells suspended in the specially formulated MACS GMP Tyto Running Buffer are loaded into the input chamber, which contains an adjustable propeller to keep cells in suspension during the sort.
After loading the cartridge into the instrument, filtered air is applied to the input chamber through a pressure inlet. Driven by very low air pressure (~3 psi), fluorescently labeled cells flow from the input chamber, via a microchannel, through the microchip. Within the microchip, cells are interrogated by three lasers. Fluorescent and scatter light signatures are used to select target cells, which are then redirected by a sort valve located within the microchannel.
To redirect a target cell into the positive collection chamber, a magnetic field is applied to the microchip. This triggers the sorting valve to open and therefore changes the flow of the target cell towards the positive collection chamber. After a target cell has passed through, a silicon spring returns the valve into its original position.
Unlike during conventional droplet sorting procedures, the cells do not experience high pressure, they are not decompressed, and no charge is applied, resulting in great viability and functionality of cells.
