LS Columns are designed for positive selection of cells. They are also suitable for the depletion of strongly magnetically labeled cells.
LS Columns can be used with the following separators:
MidiMACS™ Separator
QuadroMACS™ Separator
VarioMACS™ Separator, in combination with an LS Column Adapter
SuperMACS™ II Separator
MultiMACS Cell24 Separator Plus, in combination with the Single Column Adapter
Column capacity
For lymphoid cells from blood, bone marrow or tissue:
Up to 10
8
magnetically labeled cells from up to 2×10
9
total cells.
For the capacity of cells from other tissues, e.g., neural tissues, please refer to the data sheet provided with the particular MACS Cell Separation Reagent.
For use with the MultiMACS Cell24 Separator Plus:
Up to 10
8
magnetically labeled cells from up to 1×10
9
total cells.
Background information
The matrix of the LS Columns is composed of ferromagnetic spheres, which are covered with a cell-friendly coating allowing fast and gentle separation of cells.When placed in the magnetic field of a MACS
®
Separator, the spheres amplify the magnetic field by 10,000-fold, thus inducing a high gradient within the column. This is crucial for isolation of cells which are only minimally labeled with MACS
®
MicroBeads, leaving enough epitopes free for concurrent antibody staining. The space between the spheres is several times larger than primary and most cultured cells. This allows the cells to freely flow through the column. Magnetically labeled cells are held in suspension within the column and do not actually “bind” the column matrix. This suspension minimizes stress on the cells and allows for efficient sterile washing by avoiding cell aggregation.
LS Columns can be used to separate material less than 30 μm in size. The columns are individually packed in a sterile way.
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D Separation columns
LD separation columns