YILIMART - StraightFrom LRSC CD3 MicroBead Kit

The StraightFrom

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MicroBead Kit was developed for the rapid positive selection of CD3

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T cells directly from one LRSC using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS

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Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. LS Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire LRSC.

Background information

The CD3 antigen is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes.

Detailed separation procedure

One entire LRSC sample is labeled with StraightFrom

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LRSC CD3 MicroBeads and loaded onto LS Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

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Separator. After removing the column from the magnetic field, the magnetically retained CD3

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T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD3

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cells are well suited for further flow cytometric, functional, or molecular analysis.

Figure 1

Separation of an LRSC sample using the StraightFrom

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LRSC CD3 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and LS Columns. Cells were fluorescently stained with REAfinity™ Antibodies CD3-FITC, CD14‑VioGreen™, as well as CD45‑VioBlue

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and analyzed by flow cytometry using the MACSQuant

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Analyzer. Viable leukocytes were gated for analysis based on scatter signals, propidium iodide fluorescence, and CD45 expression.