Background information
YPet is an improved version of yellow fluorescent protein (YFP) that offers enhanced brightness, reduced pH sensitivity, and faster folding kinetics.
1 StemMACS YPet mRNA has been designed for transient expression of the YPet protein after transfection. Expression can be easily detected by fluorescence microscopy or flow cytometry. The absorption maximum of YPet is at 517 nm, its emission maximum at 530 nm.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
