YILIMART - Mouse IL-2 IS, premium grade

IL-2 IS stands for interleukin 2 “improved sequence” and is a variant of IL-2 with the serine substituted for the native cysteine at amino acid position 160.

Mouse IL-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.

Applications

Mouse IL-2 IS can be used for a variety of applications, including:

In vitro activation and propagation of mouse T cells, e.g., using the T Cell Activation/Expansion Kit, mouse.
In vitro activation and expansion of NK cells.
Generation of lymphokine-activated killer (LAK) cells.

Background information

Interleukin 2 (IL-2), a potent lymphoid cell growth factor, is a typical four α-helix bundle cytokine. IL-2 is produced by activated T cells, especially the CD4

⁺

T helper cell population. It plays an important role in both the activation and maintenance of immune responses and in lymphocyte development. IL-2 promotes proliferation and differentiation of T cells, NK cells, and B cells and is involved in the elimination of self-reactive T cells. IL-2 signals through a receptor complex consisting of IL-2 receptor α-chain (CD25), β- and common γ-chain. The later two are also used for IL-15 signaling.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation of mouse CTLL-2 cells
premium grade: ≥ 2×

10
⁷
U/mg

(typical activity: ≥ 2×

10
⁷
U/mg

)
research grade: ≥ 8×

10
⁶
U/mg

Figure 1
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Figure 1

SDS-PAGE of Mouse IL-2 IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Mouse IL-2 IS, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 2
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Figure 2

Mass spectrometry analysis (ESI-MS) of Mouse IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 17213 Da.

Mass spectrometry analysis (ESI-MS) of Mouse IL-2 IS, premium grade. The peak corresponds to the calculated molecular mass of 17213 Da.
Figure 3
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Figure 3

Mouse IL-2 IS biological activity.
Activity of Mouse IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).

Mouse IL-2 IS biological activity.
Activity of Mouse IL-2 IS, premium grade, (red bar) was compared to another commercially available product (black bar).
Figure 4
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Figure 4

Mouse IL-2 IS activity assay.
The biological activity of Mouse IL-2 IS, premium grade was determined by proliferation assay using CTLL-2 cells.

Mouse IL-2 IS activity assay.
The biological activity of Mouse IL-2 IS, premium grade was determined by proliferation assay using CTLL-2 cells.