Detailed separation procedure

• Figure 1

  Untouched pan T cells were isolated from buffy coat using the MACSxpress ® Buffy Coat Pan T Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45-VioBlue ® , CD3-FITC, and CD56-PE analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.