Detailed separation procedure

• Figure 1

  Untouched pan B cells were isolated from 30 mL of LRSC sample using the MACSxpress ® LRSC Pan B Cell Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD45‑VioBlue ® , CD19-PE, and CD20‑APC and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

  Before separation	After separation