YILIMART - aIg λ Light Chain-PE, h, IS7-24C7, 30 t

Immunoglobulins produced by B cells are composed of two heavy and two light chains, connected by disulfide bonds. Differences in the composition of the heavy chain constant regions determine the isotype of the antibody. The light chains of all isotypes are either of κ or λ subtype.

Clone IS7-24C7 detects human Ig light chains of the λ subtype. Heavy chains and light chains of the κ subtype are not detected.

Technical specifications

Antigen: Ig λ Light Chain
Clone: IS7-24C7
Isotype: mouse IgG1κ
Isotype control: Mouse IgG1 – isotype control antibodies
Entrez Gene ID: 3537, 3538, 3539, 3542, 28834
Cross-reactivity:

rhesus monkey (
Macaca mulatta
)

,

cynomolgus monkey (
Macaca fascicularis
)
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: The antibody is suited for staining of formaldehyde-fixed cells.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: PE, APC-Vio770

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with Anti-Ig λ Light Chain antibodies as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant

^®

Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

Figure 2
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Figure 2

Rhesus monkey PBMCs were stained with Anti-Ig λ light chain-Biotin, Anti-Biotin-PE, and CD20-FITC antibodies and analyzed by flow cytometry.

Rhesus monkey PBMCs were stained with Anti-Ig λ light chain-Biotin, Anti-Biotin-PE, and CD20-FITC antibodies and analyzed by flow cytometry.

aIg λ Light Chain-PE, h, IS7-24C7, 30 t

For research use only

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Technical specifications

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