AstroMACS Separation Buffer removes dead cells and enhances adult astrocyte culture: Adult astrocytes were isolated from the brain of 8-week-old mice using the Adult Brain Dissociation Kit and the Anti-ACSA-2 MicroBead Kit in combination with (A) or without (B) the AstroMACS Separation Buffer.
Cells were then cultivated in AstroMACS Medium at a density of 100,000 cells /well in a 24-well Imaging Plate for 7 days. Cells were fixed and stained with Anti-GLAST antibody (green) and DAPI (blue).