YILIMART - Leukopak CD14 MicroBead Kit, human

The StraightFrom

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Leukopak

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MicroBead Kit was developed for the rapid positive selection of CD14

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cells directly from Leukopak

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using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Block and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak

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.

Background information

The CD14 antigen belongs to the LPS receptor complex. It is strongly expressed on most monocytes and macrophages and weakly on neutrophils.

Detailed separation procedure

A Leukopak

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sample is labeled with StraightFrom

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Leukopak

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CD14 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the column from the magnetic field, the magnetically retained CD14

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cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD14

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cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block

Figure 1

Separation of a Leukopak

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sample using the StraightFrom

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Leukopak

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CD14 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD14-APC, CD15-PE-Vio

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770, as well as CD45-VioBlue

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and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.