YILIMART - Leukopak CD8 MicroBead Kit, human

The StraightFrom

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Leukopak

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MicroBead Kit was developed for the rapid positive selection of CD8

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cells directly from Leukopak

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using the MultiMACS™ Cell24 Separator Plus for semi-automated separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, or cell count. Multi-24 Column Block and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a 1/2 Leukopak

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.

Background information

In blood, the CD8 antigen is expressed on human cytotoxic T cells and on a subset of CD16

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NK cells. The CD8 antigen acts as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on CD8

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cytotoxic T cells.

Detailed separation procedure

A Leukopak

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sample is labeled with StraightFrom

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Leukopak

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CD8 MicroBeads and loaded onto Multi-24 Column Block, which is placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

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Separator. After removing the column from the magnetic field, the magnetically retained CD4

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T cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD8

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cells are well suited for further flow cytometric, functional, or molecular analysis.

Columns

Multi-24 Column Block

Figure 1

Separation of a Leukopak

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sample using the StraightFrom

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Leukopak

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CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Multi-24 Column Block. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue

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and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.