Background information

Detailed separation procedure

Downstream applications

• Figure 1

  Separation of a buffy coat sample using the StraightFrom ® Buffy Coat CD8 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD3-PE, CD8‑APC, as well as CD45‑VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

  Analysis gate	CD8 + cells

• Brochures and posters

  StraightFrom

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  MicroBeads - Cell isolation directly from blood

• Scientific posters

  Isolation of functional cells straight from buffy coat, LRSC, and Leukopak

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  . Schlemminger, M. et al. SLAS (2020)