YILIMART - Buffy Coat CD19 MicroBead Kit, human

The StraightFrom

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Buffy Coat CD19 MicroBead Kit has been developed for the rapid positive selection of CD19

⁺

cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS

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Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat.

Background information

The CD19 antigen is expressed from the early pro–B cell to the B cell blast stage. It is also present on most malignant B cells. CD19 expression is down-regulated upon terminal differentiation of B cells to plasma cells.

Detailed separation procedure

One entire buffy coat is labeled with StraightFrom

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Buffy Coat CD19 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS

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Separator. After removing the column from the magnetic field, the magnetically retained CD19

⁺

cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD19

⁺

cells are well suited for further flow cytometric, functional, or molecular analysis.

Figure 1

Separation of a buffy coat sample using the StraightFrom

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Buffy Coat CD19 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. Cells were fluorescently stained with CD19-PE, CD20‑APC, as well as CD45-VioBlue

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and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Analysis gate	CD19 ⁺ cells

Brochures and posters

StraightFrom
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MicroBeads - Cell isolation directly from blood
Scientific posters

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak
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. Schlemminger, M. et al. SLAS (2020)

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Buffy Coat CD19 MicroBead Kit, human

For research use only

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Background information

Detailed separation procedure

Downstream applications

Figure 1

Brochures and posters

Scientific posters

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