YILIMART - Human Oncostatin M IS, rg (10 μg)

Human Oncostatin M IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays. IS stands for "improved sequence" and is a variant of Human Oncostatin M with a proprietary amino acid substitution.

Applications

Human Oncostatin M IS can be used for a variety of applications including:

Study of hematopoiesis, osteogenesis, and neurogenesis.
Expansion of human fibroblasts.

Alternative names

OSM

Background information

Oncostatin M, also known as OSM, is a member of the interleukin 6 (IL-6) related cytokine subfamily that includes IL-6, interleukin 11 (IL-11), leukemia inhibitory factor (LIF), and granulocyte colony-stimulating factor (G-CSF). It is a growth and differentiation factor expressed by activated T cells and monocytes that is involved in hematopoiesis, osteogenesis, and neurogenesis. Oncostatin M induces the expression of cytokines like G-CSF and GM-CSF by endothelial cells. It inhibits the growth of a number of tumor cell lines and promotes the growth of human fibroblasts and smooth muscle cells. Human Oncostatin M is active on murine cells.

Quality description

Research-grade

cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.

Premium-grade

cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific certificates of analysis are available on request (macstec@miltenyibiotec.de).

Biological activity

Proliferation assay using TF-1 cells (NIBSC 93/564)
premium grade: ≥ 2×

10
⁷
U/mg
research grade: ≥ 8×

10
⁶
U/mg

Figure 1
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Figure 1

Mass spectrometry analysis (ESI-MS) of Human Oncostatin M IS, premium grade. The peak corresponds to the calculated molecular mass of 22044 Da.

Mass spectrometry analysis (ESI-MS) of Human Oncostatin M IS, premium grade. The peak corresponds to the calculated molecular mass of 22044 Da.
Figure 2
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Figure 2

SDS-PAGE of Human Oncostatin M IS, premium grade
under reduced (R) and non-reduced (NR) conditions.

SDS-PAGE of Human Oncostatin M IS, premium grade
under reduced (R) and non-reduced (NR) conditions.
Figure 3
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Figure 3

Human Oncostatin M IS biological activity.
Activity of Human Oncostatin M IS, premium grade (red bar) was compared to another commercially available product (black bar).

Human Oncostatin M IS biological activity.
Activity of Human Oncostatin M IS, premium grade (red bar) was compared to another commercially available product (black bar).
Figure 4
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Figure 4

Human Oncostatin M IS activity assay.
The biological activity of Human Oncostatin M IS, premium grade, was determined by prolifertaion of TF-1 cells.

Human Oncostatin M IS activity assay.
The biological activity of Human Oncostatin M IS, premium grade, was determined by prolifertaion of TF-1 cells.

Selected references
1. Kitamura, T. et al. (1989) Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell. Physiol. 140: 323-334
Certificates

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Human Oncostatin M IS, rg (10 μg)

For research use only

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Applications

Alternative names

Background information

Quality description

Biological activity

Figure 1

Figure 1

Figure 2

Figure 2

Figure 3

Figure 3

Figure 4

Figure 4

Selected references

Certificates

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