YILIMART - REA control-PE, REA293, 150ug

The REA Control antibody clone REA293 is a universal isotype control that can be used with all recombinant engineered REAfinity™ Antibodies. REAfinity Antibodies have been engineered for their high specificity and contain human IgG1 parts for constant regions. Although REAfinity Antibodies show virtually no binding to Fc receptors, the use of the clone REA293 is still recommended to control for other non Fc receptor–mediated non-specific binding of fluorochrome-conjugated REAfinity Antibodies to cells. Unspecific interactions of the fluorochrome can also be controlled with conjugated versions of clone REA293.

Alternative names

keyhole limpet hemocyanin

Technical specifications

Antigen: KLH
Clone: REA293
Isotype: recombinant human IgG1
Alternative names of antigen: keyhole limpet hemocyanin
Available conjugates: Biotin, FITC, VioBright FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Vio515

Figure 1

Splenocytes from C57BL/6 mice were stained with REA Control antibodies or with the corresponding Anti-Ly49H antibodies (left images) as well as with CD49b (DX5) antibodies. Flow cytometry was performed using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

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REA control-PE, REA293, 150ug

For research use only

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Alternative names

Technical specifications

Figure 1

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