YILIMART - REA control (S)-APC, REA293, 100 tests

The REA Control (S) antibody clone REA293 is a universal isotype control that can be used with all recombinant engineered antibodies (REAfinity™ Antibodies) that recognize cell surface antigens. REAfinity Antibodies have been engineered for their high specificity and contain human IgG1 parts for constant regions. Although REAfinity Antibodies show virtually no binding to Fc receptors, the use of the clone REA293 is still recommended to control for other non Fc receptor–mediated non-specific binding of fluorochrome-conjugated REAfinity Antibodies to cells. Unspecific interactions of the fluorochrome with the cell surface can also be controlled for with conjugated versions of clone REA293.

Alternative names

keyhole limpet hemocyanin

Technical specifications

Antigen: KLH
Clone: REA293
Isotype: recombinant human IgG1
Alternative names of antigen: keyhole limpet hemocyanin
Product format: Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioBright FITC, PE, APC, VioBlue, VioGreen, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin, VioBright 667, FITC

Figure 1

Human peripheral blood mononuclear cells (PBMCs) were stained with REA Control (S) antibodies or with the corresponding CD56 (clone REA196) antibodies (left images) as well as with CD3 antibodies. Flow cytometry was performed with the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

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REA control (S)-APC, REA293, 100 tests

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Alternative names

Technical specifications

Figure 1

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