YILIMART - Mouse IgG1-VioGreen, IS5-21F5, 100 tests

Mouse IgG1-VioGreen, IS5-21F5, 100 tests

For research use only

货号：: 130-113-207
描述：: Mouse IgG1-VioGreen
包装：: for 100 tests

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The Mouse IgG1 isotype control antibody clone IS5-21F5 is specific for keyhole limpet hemocyanin (KLH). This protein is not expressed on human cells or cell lines. Therefore, the antibody clone IS5-21F5 can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG1 fluorochrome-conjugated antibodies to human cells, for example via Fc receptors, or due to interactions of the fluorochrome with the cell surface.

Alternative names

keyhole limpet hemocyanin

Technical specifications

Antigen: KLH
Clone: IS5-21F5
Isotype: mouse IgG1κ
Alternative names of antigen: keyhole limpet hemocyanin
Product format: Antibodies are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: FITC, VioBright FITC, PE, APC, VioBlue, VioGreen, PerCP, PE-Vio615, PE-Vio770, APC-Vio770, PerCP-Vio700, VioBright 515, Biotin

Figure 1

Human peripheral blood lymphocytes (PBLs) were stained with Mouse IgG1 isotype control antibodies and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Gates were set on viable lymphocytes.