YILIMART - Hoechst 33342 Staining Solution

The Hoechst 33342 Staining Solution is a ready-to-use reagent for the identification of nucleated cells by flow cytometric analysis. Hoechst 33342 is widely used for enumeration of nucleated cells and for determination of DNA content in cell cycle analysis.

Hoechst 33342 is also frequently used as a nuclear counterstain in fluorescence microscopy assays.

Background information

Hoechst 33342 is a cell permeable nucleic acid stain that binds to minor groove A-T rich regions of double-stranded DNA. Therefore, the staining intensity differs between different cell types. All nucleated cells are Hoechst positive.

The fluorescence emission maximum for DNA-bound Hoechst 33342 is about 461 nm. With an excitation maximum of 355 nm it can be excited by a ultraviolet (UV) laser as well as by a violet laser (405 nm) and detected within the blue fluorescence channel, e.g., channel V1 on the MACSQuant

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Analyzer 10.

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10⁶ human peripheral blood mononuclear cells (PBMCs, 8 days old) were stained with the Hoechst 33342 Staining Solution, incubated for 15 minutes in the dark at room temperature, and analyzed by flow cytometry using the MACSQuant Analyzer 10. Cell debris was excluded from the analysis based on scatter signals. Nucleated cells are positive for Hoechst 33342 and belong to the P2 population.

10⁶ human peripheral blood mononuclear cells (PBMCs, 8 days old) were stained with the Hoechst 33342 Staining Solution, incubated for 15 minutes in the dark at room temperature, and analyzed by flow cytometry using the MACSQuant Analyzer 10. Cell debris was excluded from the analysis based on scatter signals. Nucleated cells are positive for Hoechst 33342 and belong to the P2 population.

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Hoechst 33342 Staining Solution

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