The 7-AAD Staining Solution is a ready-to-use reagent suitable for the exclusion of dead and apoptotic cells from flow cytometric analysis.
Background information
7-AAD (7-amino-actinomycin D) is a fluorescent dye that intercalates into double-stranded DNA (GC rich regions). It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells. Therefore, it can be used instead of propidium iodide (PI) for the evaluation of cell death and apoptosis.
The fluorescence emission maximum for 7-AAD is at 647 nm. When excited at 488 nm, 7-AAD is detected in the red fluorescence channel commonly used for R-phycoerythrin (PE)-Cy
®
5 tandem dye detection, with minimal spectral overlap into the yellow fluorescence channel commonly used for PE detection.
10⁶ human peripheral blood mononuclear cells (PBMCs, 8 days old) were stained with 7-AAD Staining Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris was excluded from the analysis based on scatter signals. Dead cells are positive for 7-AAD and thus can be excluded from analysis. Viable cells belong to the P2 population.
10⁶ human peripheral blood mononuclear cells (PBMCs, 8 days old) were stained with 7-AAD Staining Solution and analyzed by flow cytometry using the MACSQuant
®
Analyzer 10. Cell debris was excluded from the analysis based on scatter signals. Dead cells are positive for 7-AAD and thus can be excluded from analysis. Viable cells belong to the P2 population.
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