Background information

Applications

• Figure 1

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  Figure 1

  Human T cells were transduced with gamma-retroviral vectors encoding GFP in the presence of Vectofusin-1. Transduction was performed either at 37 °C in an incubator (static), or the T cells were centrifuged at 400×g for 2 hours at 32 °C (spin) before transfer to 37 °C. GFP expression was analyzed on day 7 of cultivation using a MACSQuant

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  Analyzer. T cell viability was assessed by flow cytometry using Viobility™ Fixable Dye.

  
  Human T cells were transduced with gamma-retroviral vectors encoding GFP in the presence of Vectofusin-1. Transduction was performed either at 37 °C in an incubator (static), or the T cells were centrifuged at 400×g for 2 hours at 32 °C (spin) before transfer to 37 °C. GFP expression was analyzed on day 7 of cultivation using a MACSQuant

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  Analyzer. T cell viability was assessed by flow cytometry using Viobility™ Fixable Dye.

• Selected references

  1. Fenard, D. et al. (2013) Infectivity enhancement of different HIV-1-based lentiviral pseudotypes in presence of the cationic amphipathic peptide LAH4-L1. J. Virol. Methods 189(2): 375-378
  2. Ingrao, D. et al. (2014)
     Concurrent measures of fusion and transduction efficiency of primary CD34

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     cells with human immunodeficiency virus 1-based lentiviral vectors reveal different effects of transduction enhancers.
     Hum Gene Ther Methods 25(1): 48-56

• Scientific posters

  Automated clinical-scale retroviral transduction of T cells in a functionally closed system
  Effective retroviral transduction of primary T cells and hematopoieticstem cells using the soluble transduction enhancer: Vectofusin-1

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