YILIMART - Whole Blood CD326 (EpCAM) MB,human 5x2ml

StraightFrom

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Whole Blood CD326 (EpCAM) MicroBeads, human were developed for rapid positive selection of CD326

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cells directly from whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom

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Whole Blood CD326 (EpCAM) MicroBeads have been developed for the positive selection of human epithelial cells directly from anticoagulated whole blood by using the autoMACS

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Pro Separator or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Epithelial cell adhesion molecule (EpCAM), which is also known as CD326 and human epithelial antigen (HEA), is broadly expressed on cells of epithelial origin and on epithelial derived tumor cells.

StraightFrom Whole Blood CD326 (EpCAM) MicroBeads are used for the positive selection of viable epithelial tumor cells from peripheral blood of patients with carcinomas.

Columns

autoMACS

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Columns or Whole Blood Columns.

Figure 1

Enrichment of tumor cells from whole blood sample spiked with cells from a breast cancer cell line (SK-BR3) using StraightFrom

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Whole Blood CD326 (EpCAM) MicroBeads and the program “Posselwb” on the autoMACS

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Pro Separator. Cells are fluorescently stained with CD326 (EpCAM)-FITC and CD45-VioBlue

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and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	After separation

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StraightFrom
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MicroBeads - Cell isolation directly from blood

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Whole Blood CD326 (EpCAM) MB,human 5x2ml

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Figure 1

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