Sample Preparation
Cell Separation
Flow Cytometry
Cell Culture&Stimulation
Cell manufacturing
Imaging and Microscopy
MACSmolecular
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Enrichment of tumor cells from whole blood sample spiked with cells from a breast cancer cell line (SK-BR3) using StraightFrom ® Whole Blood CD326 (EpCAM) MicroBeads and the program “Posselwb” on the autoMACS ® Pro Separator. Cells are fluorescently stained with CD326 (EpCAM)-FITC and CD45-VioBlue ® and analyzed by flow cytometry using the MACSQuant ® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
Before separation | After separation |