The different T helper (Tʜ) cell subsets have a central function in initiation, programming, and regulation of the various protective and pathological antigen-specific immune responses. T helper 1 (Tʜ1) cells are effective against intracellular pathogens and tumor cells and are also involved in immunological disorders, such as autoimmune reactions.
In vitro , Tʜ1 cells can be generated from naive CD4
+ T cells upon primary activation via T cell receptor in the presence of IL-12. Addition of IL-2 will enhance T cell proliferation. To inhibit polarization towards Tʜ2, the Anti-IL-4 pure – functional grade antibody is used.
