YILIMART - StemMACS Flp Recombinase mRNA

StemMACS™ Flp Recombinase mRNA encodes a codon optimized version of Flp recombinase.

Background information

Flippase (Flp) is a site-specific recombinase derived from the yeast

Saccharomyces cerevisiae

. It mediates recombination between two identical flippase recognition target (FRT) sites.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Figure 1

Flp-mediated recombination in mouse ES cells:

HM1 mouse embryonic stem cells carrying a GFP reporter gene flanked by two FRT sites were electroporated with 15 µg StemMACS Flp Recombinase mRNA and analyzed by flow cytometry 96h after transfection. 88% of all cells were GFP-negative indicating successful Flp-mediated recombination.

Transfection control	Flp-transfected cells

Selected references
1. Raymond, C.S. et al. (2007) High-efficiency FLP and PhiC31 site-specific recombination in mammalian cells. PLoS One 2(1): e162

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StemMACS Flp Recombinase mRNA

For research use only

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Background information

Detailed procedure

Quality description

Figure 1

Selected references

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