YILIMART - WB and BM CD138 MicroBeads, human

StraightFrom

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Whole Blood and Bone Marrow CD138 MicroBeads have been developed for the positive selection of CD138

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cells directly from anticoagulated whole blood or bone marrow by using the autoMACS

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Pro Separator or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

The CD138 antigen, also known as syndecan-1, is primarily found on normal and malignant plasma cells in the bone marrow. In the peripheral blood of healthy donors approximately 50% of plasma cells express this antigen. The CD138 antigen is not expressed by naive B cells, germinal center B cells, memory B cells, T cells, or monocytes.

Detailed separation procedure

Anticoagulated cell samples of 0.25–15 mL are labeled with StraightFrom

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Whole Blood and Bone Marrow CD138 MicroBeads and subsequently separated with the autoMACS

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Pro Separator or using the Whole Blood Column Kit. StraightFrom Whole Blood and Bone Marrow MicroBeads in combination with the autoMACS Pro Separator allow standardization of the cell separation procedure and safe handling of hazardous samples.

Downstream applications

Isolation of CD138

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cells from whole blood or bone marrow. The purified CD138

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cells are well suited for further flow cytometric, functional, or molecular analysis such as FISH, PCR analysis, microarrays, and protein analysis, and for analysis of cell surface markers or intracellular antigens, for example immunoglobulin light chains.

Columns

autoMACS

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Columns or Whole Blood Columns.

Figure 1

Separation of a whole blood sample spiked with U266 cell line (0.5%) using the StraightFrom

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Whole Blood and Bone Marrow CD138 MicroBeads, the Whole Blood Column Kit, and an MS Column. Cells were fluorescently stained with CD45-FITC, CD138-PE, and CD19-APC and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris, dead cells, and CD45

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cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.