StemMACS™ IWP-2 is a selective small molecule inhibitor of Wnt signaling.
Background information
StemMACS™ IWP-2 inactivates the membrane-bound O-acyltransferase Porcupine (Porcn) which mediates palmitylation of Wnt proteins. Inhibition of Porcn prevents Wnt secretion and thus blocks activation of the Wnt signaling pathway. Consistent with the missing Wnt ligand, levels of phosphorylated Lrp6 and Dvl2 are decreased in the presence of IWP-2 and β-catenin cannot accumulate.
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Applications
Published applications for IWP-2 include:
Differentiation of human ES and iPS cells into cardiac mesoderm2
Chen, B. et al. (2009) Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. Nat. Chem. Biol. 5: 100-107
Lian et al. (2013) Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions. Nat. Protoc. 8(1): 162-175
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