YILIMART - Rat IgM-VioBright FITC 200 tests

Rat IgM-VioBright FITC 200 tests

For research use only

货号：: 130-105-158
描述：: Rat IgM-VioBright FITC
包装：: 30 µg in 1 mL

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The Rat IgM isotype control antibody clone ES26-13D3.4 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on mammalian cells or cell lines. Therefore, the antibody clone ES26-13D3.4 can be used as a negative control to distinguish specific from non-specific binding of Rat IgM fluorochrome-conjugated antibodies to human and mouse cells, for example, via Fc receptors, or due to interactions of the fluorochrome with the cell surface.

Alternative names

keyhole limpet hemocyanin

Technical specifications

Antigen: KLH
Clone: ES26-13D3.4
Isotype: rat IgMκ
Alternative names of antigen: keyhole limpet hemocyanin
Available conjugates: PE, APC, PE-Vio770, APC-Vio770, PerCP-Vio700, Biotin, FITC, VioBright FITC

Figure 1

Splenocytes from Balb/c mice were stained with Rat IgM antibodies or with the corresponding CD25 antibodies (left images) and analyzed by flow cytometry using MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.