YILIMART - Whole Blood CD66b MicroBeads, human

StraightFrom

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Whole Blood CD66b MicroBeads were developed for rapid positive selection of CD66b

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cells directly from human whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.

Background information

StraightFrom

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Whole Blood CD66b MicroBeads are developed for the positive selection of CD66b

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cells directly from anticoagulated whole blood by using the autoMACS

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Pro Separator, the MultiMACS™ Cell24 Separator Plus, or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. CD66b is a 95–100 kDa glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. It is expressed on neutrophils and eosinophils but not on basophils and lymphocytes.

Detailed separation procedure

Anticoagulated whole blood samples of 0.25–15 mL are labeled with StraightFrom

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Whole Blood CD66b MicroBeads to be subsequently separated with the autoMACS

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Pro Separator, the MultiMACS™ Cell24 Separator Plus, or using the Whole Blood Column Kit.

Columns

autoMACS

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Columns or Whole Blood Columns.

Figure 1

Separation of a whole blood sample using the StraightFrom

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Whole Blood CD66b MicroBeads and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns without washing step after labeling. Cells were fluorescently stained with CD15‑FITC, CD66b-PE, as well as CD45‑VioBlue

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and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cells were triggered via CD45-VioBlue, cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	After separation

Brochures and posters

StraightFrom
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MicroBeads - Cell isolation directly from blood

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Whole Blood CD66b MicroBeads, human

For research use only

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Background information

Detailed separation procedure

Columns

Figure 1

Brochures and posters

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