YILIMART - StemMACS mRNA Transfection Kit

The StemMACS™ mRNA Transfection Kit is a lipid-based transfection system designed for efficient mRNA delivery into various cell types. The optimized formulation ensures high transfection efficiency at minimal cytotoxicity.

Background information

The transient, non-integrative expression of key developmental regulators, recombinases or markers via mRNA transfection is a powerful tool for modulating cell fate.

The StemMACS™ mRNA Transfection Reagent has been selected for its minimal cytotoxicity and high transfection efficiency. It is therefore particularly suited for sensitive cell lines such as pluripotent stem cells and complex transfection schedules that involve repeated mRNA delivery over several days. The StemMACS™ mRNA Transfection Kit has been successfully used for transfection of primary human fibroblasts and the generation of iPS cell lines by mRNA reprogramming.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Applications

Transient, non-integrative delivery of mRNA encoded factors into a broad range of cell types, including primary fibroblasts and human iPS cells
mRNA reprogramming of human fibroblasts
mRNA-induced differentiation of stem and progenitor cells
mRNA-induced transdifferentiation of differentiated cells
mRNA-induced recombination
Transient labeling with fluorescent proteins

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Figure

Primary human fibroblasts of different origin were plated at 2.5 x10
⁴
cells/well in a 24-well plate. The next day, cells were transfected with the indicated amounts of StemMACS eGFP mRNA and 0.6 µL of StemMACS mRNA Transfection Reagent. The percentage of GFP expressing cells (green bars) and the mean GFP fluorescence among living cells (blue diamonds) was determined 24 h post transfection. Cell viability was >95% in all samples.

Primary human fibroblasts of different origin were plated at 2.5 x10
⁴
cells/well in a 24-well plate. The next day, cells were transfected with the indicated amounts of StemMACS eGFP mRNA and 0.6 µL of StemMACS mRNA Transfection Reagent. The percentage of GFP expressing cells (green bars) and the mean GFP fluorescence among living cells (blue diamonds) was determined 24 h post transfection. Cell viability was >95% in all samples.

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StemMACS mRNA Transfection Kit

For research use only

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Background information

Detailed procedure

Applications

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