Detailed separation procedure

• Figure 1

  Untouched neutrophils were isolated from 8 mL of human EDTA anticoagulated whole blood using the MACSxpress ® Whole Blood Neutrophil Isolation Kit, a MACSmix™ Tube Rotator, and a MACSxpress Separator. The isolated cells were fluorescently stained with CD14-PerCP, CD15-PE, CD16-APC, CD45-VioBlue ® , and CD193-FITC and analyzed by flow cytometry using the MACSQuant ® Analyzer 10. Cell debris, non-leukocytes, and dead cells were excluded from the analysis based on CD45 expression, scatter signals, and propidium iodide fluorescence.

  Unseparated fraction

  After separation

• Scientific posters

  Untouched isolation of functionally unaffected neutrophils from whole blood within 20 minutes