YILIMART - StemMACS NeuroG2 mRNA, human

StemMACS™ NeuroG2 mRNA encodes the human transcription factor Neurogenin 2 for transient transfection.

Applications

Neuronal differentiation of pluripotent stem cells^1,2
Conversion of fibroblasts into induced neuronal (iN) cells³

Background information

Neurogenin 2, encoded by the human NeuroG2 (NGN2) gene, is a neural-specific basic helix-loop-helix (bHLH) transcription factor that regulates specification of neuronal fate in ectodermal cells. Neurogenin 2 is expressed in neural progenitor cells within the developing central and peripheral nervous systems. In addition, it plays a role in the differentiation and survival of midbrain dopaminergic neurons. In pluripotent stem cell research, ectopic expression of Neurogenin 2 has been used to promote neuronal differentiation of ES or iPS cells

^1,2

and to convert fibroblasts into induced neuronal (iN) cells

³

.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Figure 1

Transfection control

NeuroG2-transfected cells

Selected references
1. Thoma, E.C. et al. (2012) Ectopic expression of neurogenin 2 alone is sufficient to induce differentiation of embryonic stem cells into mature neurons. PLoS One 7(6): e38651
2. Zhang, Y. et al. (2013) Rapid single-step induction of functional neurons from human pluripotent stem cells. Neuron 78(5): 785-798
3. Liu, M.L. et al. (2013) Small molecules enable neurogenin 2 to efficiently convert human fibroblasts into cholinergic neurons. Nat Commun 4: 2183

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StemMACS NeuroG2 mRNA, human

For research use only

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Applications

Background information

Detailed procedure

Quality description

Figure 1

Selected references

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Transfection control	NeuroG2-transfected cells