YILIMART - StemMACS NeuroD1 mRNA, human

StemMACS™ NeuroD1 mRNA encodes the neural transcription factor NeuroD1 for transient transfection.

Applications

Neuronal differentiation of pluripotent stem cells^1,2
Conversion of fibroblasts into induced neuronal (iN) cells³

Background information

NeuroD1 is a member of the basic helix-loop-helix (bHLH) family of transcription factors. It forms heterodimers with other bHLH proteins and activates transcription by binding to the E-box motif. NeuroD1 is a regulator of insulin expression. In pluripotent stem cell research, ectopic expression of NeuroD1 has been used to induce neuronal differentiation.

Detailed procedure

For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description

StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.

StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Figure 1

NeuroD1 expression after mRNA transfection:

Human BJ fibroblasts were transfected with 150 ng StemMACS™ NeuroD1 mRNA and 75 ng StemMACS Nuclear eGFP mRNA. 24 hours post transfection, NeuroD1 expression (red) and colocalization with nuclear eGFP (green) were analyzed by immunofluorescence microscopy.

Transfection control	NeuroD1-transfected cells

Selected references
1. Pang et al. (2011) Induction of human neuronal cells by defined transcription factors. Nature 476: 220-223

查看数据文件查看下载

StemMACS NeuroD1 mRNA, human

For research use only

您浏览过的商品

Applications

Background information

Detailed procedure

Quality description

Figure 1

Selected references

相关产品