Background information
The homeobox and LIM-domain containing protein Lmx1a acts as a positive regulator of insulin gene transcription. In addition, Lmx1a plays a role in the development of dopamine-producing neurons during embryogenesis. In pluripotent stem cell research, overexpression of Lmx1a in combination with other transcription factors (e.g. Mash1, Nurr1) has been used to accelerate the differentiation into functional dopaminergic neurons.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
