Background information
CCAAT/enhancer-binding protein beta (C/EBP-beta) is a member of the basic leucine zipper (bZIP) containing family of transcription factors. It can function as a homodimer or in heterodimeric complexes with other C/EBP family members, such as C/EBPalpha, -beta or -gamma. C/EBP-beta plays a role in the regulation of genes that are important for immune and inflammatory responses. In addition, C/EBP-beta is transiently induced during the early stages of adipocyte differentiation and promotes adipogenesis in cooperation with other adipogenic factors such as PPAR-gamma. In pluripotent stem cell research overexpression of C/EBP-beta has been used to induce the differentiation into adipose tissue.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
