Background information
The basic helix-loop-helix (BHLH) transcription factor Mash1(Ascl1) dimerizes with other BHLH family members to activate transcription via recognition of the E-box motif (5’-CANNTG-3’). In pluripotent stem cell biology, Mash1 plays a role in neuronal commitment and differentiation, e.g. in the generation of olfactory and autonomic neurons.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
