Background information

Applications

• Figure 1

  Pluripotent stem cells cultured in StemMACS iPS-Brew XF display typical colony morphology.

• Figure 2

  Cells cultured in StemMACS iPS-Brew XF maintain full pluripotent differentiation potential and can form teratomas containing derivatives of all three germ layers.

  Endoderm	Ectoderm
  Mesoderm

• Figure 3

  Human iPS cells cultured in StemMACS iPS-Brew XF show high expression of pluripotency markers.

  Clone 1
  Clone 2

• Selected references

  1. Kirkeby, A. et al. (2017) Predictive markers guide differentiation to improve graft outcome in clinical translation of hESC-based therapy for Parkinson’s Disease. Cell Stem Cell 20: 1-14
  2. Lorenz, C. et al. (2017) Human iPSC-derived neural progenitors are an effective drug discovery model for neurological mtDNA disorders. Cell Stem Cell 20(5): 659-674
  3. Mitzelfeld, K. et al. (2016) The human 343delT HSPB5 chaperone associated with early-onset skeletal myopathy causes defects in protein solubility. J. Biol. Chem. 291(29): 14939-14953
  4. Giobbe, G.G. et al. (2015) Functional differentiation of human pluripotent stem cells on a chip. Nat. Methods 12: 637-640
  5. Park, C.-Y. et al. (2016) Modeling and correction of structural variations in patient-derived iPSCs using CRISPR/Cas9 Nat. Protoc. 11: 2154-2169
  6. Solbrant, S. et al. (2017) Generation of high-purity human ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation Nat. Protoc. 12(9): 1962-1979
  7. Es-Salah-Lamoureux, Z. et al. (2016) HIV-Tat induces a decrease in IKr and IKs via reduction in phosphatidylinositol-(4,5)-bisphosphate availability. J. Mol. Cell. Cardiol. 99: 1-13
  8. Si-Tayeb, K. et al. (2016) Urine‑sample‑derived human induced pluripotent stem cells as a model to study PCSK9‑mediated autosomal dominant hypercholesterolemia. Dis Model Mech 9(1): 81-90

• Brochures and posters

  Cell culture reagents for your stem cell research
  StemMACS iPS-Brew XF

• Scientific posters

  Standardized QC assays and automated expansion of PSCs
  A versatile and robust xeno- and serum-free cultivation system for human pluripotent stem cells (Poster, ISSCR 2014)

• Customer reports and application notes

  StemMACS™ iPS-Brew enables flexible feeding schedules
  Performance of StemMACS™iPS-Brew XF in the maintenance and neural differentiation of human pluripotent stem cells. Cheng, X. et al. (2017)
  Multicolor flow cytometry analysis of human pluripotent stem cell cultures

• Further downloads

  StemMACS iPS-Brew XF - FAQ