The StemMACS MSC Expansion Media Kit is an optimized and standardized serum- and xeno-free medium for the reproducible and reliable expansion and enumeration of MSCs from human BM samples or other tissue sources, while maintaining the cells' multi-lineage differentiation potential. StemMACS MSC Expansion Media Kit XF is completely free of non-human animal derived components and does not require the use of cell attachment substrates.
Detailed procotols for MSC culture can be found on the Library tab.
Background information
The StemMACS MSC Expansion Media Kit XF is an optimized and standardized serum-free and xeno-free medium for the reproducible and reliable generation and expansion of mesenchymal stem cells (MSCs) from human bone marrow (BM) samples or other tissue sources. As MSCs appear at relatively low frequency in human BM samples and other tissues, their
in vitro
propagation is often necessary in order to obtain sufficient cell numbers for further experiments, such as
in vivo
transplantation studies in animals or
in vitro
differentiation or functional characterization. The formulation of StemMACS MSC Expansion Media Kit XF was designed to efficiently support the expansion of MSCs
in vitro
while maintaining their differentiation potential. The media formulation is xeno- and serum-free and is manufactured under strictly controlled conditions using ingredients of the highest quality. StemMACS Expansion Media Kit XF offers consistent lot-to-lot performance and optimal conditions for the cultivation of MSCs. It is suitable for the derivation, expansion, and enumeration using the colony-forming–unit fibroblast (CFU-F) assay and does not require the use of any cell attachment substrate for optimal performance.
MSCs cultured in StemMACS MSC Expansion Media XF exhibit immunomodulating properties. The immunomodulation was measured using the MSC Suppression Inspector by cell proliferation of responder T (Tresp) cells in co-culture with MSCs.
MSCs cultured in StemMACS MSC Expansion Media XF exhibit immunomodulating properties. The immunomodulation was measured using the MSC Suppression Inspector by cell proliferation of responder T (Tresp) cells in co-culture with MSCs.
MSCs cultured in StemMACS MSC Expansion Media XF show an increased clonogenic potential (A) as well as cell proliferation rate (B) compared to MSCs cultured in αMEM + 5% platelet lysate or when cultured in 10% fetal calf serum (FCS)–containing media.
MSCs cultured in StemMACS MSC Expansion Media XF show an increased clonogenic potential (A) as well as cell proliferation rate (B) compared to MSCs cultured in αMEM + 5% platelet lysate or when cultured in 10% fetal calf serum (FCS)–containing media.
Human MSCs cultured in StemMACS MSC Expansion Media XF were differentiated towards osteogenic (A), adipogenic (B), or chondrogenic (C) lineage using StemMACS MSC Diff Media. Cells cultured in StemMACS MSC Expansion Media XF maintain the broad differentiation potential.
Human MSCs cultured in StemMACS MSC Expansion Media XF were differentiated towards osteogenic (A), adipogenic (B), or chondrogenic (C) lineage using StemMACS MSC Diff Media. Cells cultured in StemMACS MSC Expansion Media XF maintain the broad differentiation potential.
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