Human epidermal tissue can be dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins, which maintain the structural integrity of tissues.
In a first step, the epidermal cell layer is removed from the dermal cell layer after enzymatic treatment over night at 4 °C. The epidermal tissue is then further digested enzymatically and dissociated into a single-cell suspension by using the gentleMACS™ Dissociators.
