YILIMART - Rat IgG2a-PerCP-Vio700, 60 tests

Rat IgG2a-PerCP-Vio700, 60 tests

For research use only

货号：: 130-103-095
描述：: Rat IgG2a-PerCP-Vio700
包装：: 9 µg in 300 µL

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The rat IgG2a isotype control antibody clone ES26-15B7.3 is specific for keyhole limpet hemocyanin (KLH). This protein is not expressed on mammalian cells or cell lines. Therefore, the antibody clone ES26-15B7.3 can be used as a negative control to distinguish specific from non-specific binding of rat IgG2a fluorochrome-conjugated antibodies to human and mouse cells, for example, via Fc receptors, or due to interactions of the fluorochrome with the cell surface.

Alternative names

keyhole limpet hemocyanin

Technical specifications

Antigen: KLH
Clone: ES26-15B7.3
Isotype: rat IgG2aκ
Alternative names of antigen: keyhole limpet hemocyanin
Product format: Reagents are supplied in buffer containing stabilizer and 0.05% sodium azide.
Fixation: Cells should be stained prior to fixation, if formaldehyde is used as a fixative.
Storage: Store protected from light at 2–8 °C. Do not freeze.
Available conjugates: VioBlue, VioGreen, PerCP, PE-Vio770, PerCP-Vio700, Vio667, Biotin, FITC, VioBright FITC, PE, APC

Figure 1

Splenocytes from BALB/c mice were stained with Rat IgG2a antibodies or with the corresponding CD8a antibodies (left images). Flow cytometry was performed using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.